黄口荔枝螺转录组数据的微卫星标记开发与分析     

李威  赵姗  焦海峰  林志华  包永波 《海洋科学》2015,39(11):61-67

以黄口荔枝螺转录组测序所得到的拼接序列为基础,利用MISA软件进行微卫星分析,利用Primer5.0设计引物160对。结果显示,83对引物能够成功扩增,引物在黄口荔枝螺渔山列岛群体多态性检测中发现,37个SSR位点能够表现出多态性,一共获得了129个等位基因,各位点等位基因数为2~5个,平均每个位点等位基因数为3.49个,观测杂合度H_o,期望杂合度H_e,多态信息含量PIC范围分别介于0.000~0.800、0.146~0.645、0.139~0.573之间,有9个表现为高度多态,20个表现为中度多态,8个表现为低度多态。实验结果进行Hardy-Weinberg平衡检测后,利用Bonferroni correction法进行校正,有29个位点显著偏离,结果表明渔山列岛黄口荔枝螺群体总体遗传多样性水平较低。  相似文献   

MOLECULAR CLONING AND SEQUENCING OF A cDNA ENCODING PARTIAL PUTATIVE MOLT-INHIBITING HORMONE FROM PENAEUS CHINENSIS     

王在照  相建海 《中国海洋湖沼学报》2002,20(3):232-236

  相似文献   

Molecular cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone from Penaeus chinensis     

Wang Zai-zhao  Xiang Jian-hai 《中国海洋湖沼学报》2002,20(3):232-236

  相似文献   

Plant responses to global change: next generation biogeography     

Jeremy S. Johnson  Keith D. Gaddis  David M. Cairns  Charles W. Lafon  Konstantin V. Krutovsky 《自然地理学》2016,37(2):93-119

Global change has become a major ecological concern that has the potential to dramatically alter plant distributions and assemblages worldwide. The specific response of plants to global change will depend on their ability to move, evolve, or adjust through phenotypic plasticity. Advances in biogeographic research are on the verge of a giant leap forward if we can take advantage of emerging tools in genetics and genomics. In this review, we appraise how new, post-Sanger, high-throughput sequencing and associated technologies can be used by next generation biogeographers to assess plant responses to global change. First, we briefly review the recent advances in genome sequencing and available approaches of genomic analysis. Secondly, we discuss the three main plant responses to global change: migration, adaptation, and phenotypic plasticity. This review is intended to spark the interest of biogeographers who do not traditionally use genetics but could benefit from including genetic and genomic tools into their research. Our goal is to illustrate that their use can contribute to new perspective and add an additional level of resolution into future biogeographic research.  相似文献   

长耳珠母贝核rRNA基因ITS-2序列分析   总被引：11，自引：0，他引：11  

何毛贤  黄良民 《热带海洋学报》2004,23(5):81-84

以相应引物聚合酶链式反应(PCR)扩增长耳珠母贝(Pinctada chemnitzi)核核糖体RNA基因第2转录间隔区(ITS-2),PCR产物大小约500bp,经克隆、测序,所得序列包含5．8S和28SrRNA基因部分序列和ITS-2全序列,4种碱基含量分别为27．11％(A)、24．95％(G)、21．26％(T)和26．68％(C)。5个克隆的DNA序列差异为0．0％-0．4％,不存在个体内变异。对其潜在应用进行了讨论。  相似文献   

泥蚶(Tegillarca granosa)血细胞转录组序列中免疫相关基因的结构与mRNA表达量分析     

钟爱华  储张杰  王伟洪  牟毅 《海洋与湖沼》2020,51(2):354-363

贝类血细胞直接参与异物吞噬和包囊作用,还在伤口修复、炎症反应过程中发挥重要作用,是机体免疫防御的承担者。本研究采用RNA-seq测序技术研究了鳗弧菌感染前后泥蚶血细胞的转录组序列,通过序列组装,获得了α-葡萄糖苷酶(GAA)、α-甘露糖苷酶(LAMAN)、芳基硫酸酯酶B(ASB)、天冬酰胺氨基葡萄糖酶(AGA)、整合素β3亚基(ITB3)、热休克蛋白9(HSPA9)和Toll相互作用蛋白(TOLLIP)序列,利用Orffinder获得了其编码蛋白序列,并对蛋白序列进行了理化性质、二级结构、保守结构域和三级结构分析。结果表明:7种蛋白质二级结构均含有α螺旋、β折叠及无规则卷曲,泥蚶和人类ITB3和TOLLIP具有相同的保守结构域, Swiss-model同源建模获得的三级结构与模板同源性均超过35%(TOLLIP除外)。鳗弧菌刺激后,GAA、LAMAN、ASB、AGA和HSPA9的表达量显著增加,ITB3和TOLLIP表达量变化不明显。该研究补充和完善了对泥蚶免疫相关基因的认识,并为进一步开展泥蚶抗病机理研究提供了重要的理论依据。  相似文献   

Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing during a dinoflagellates bloom     

KAN Jinjun  CHEN Feng 《海洋学报(英文版)》2004,23(3):483-492

Dinoflagellates are unicellular eukaryotic protists that dominate in all coastal waters, and are also present in oceanic waters. Despite the central importance of dinoflagellates in global primary production, the relationship between dinoflagellates and bacteria are still poorly understood. In order to understand the ecological interaction between bacterial and dinoflageUates communities, denaturing gradient gel electrophoresis (DGGE) and SSU rRNA sequencing were applied to monitoring the population dynamics of bacteria and dinoflagellates from the onset to disappearance of a dinoflagellates bloom occurred in Baltimore Inner Harbor, from April 15 to 24, 2002. Although Prorocentrum minimum was the major bloom forming species under the light microscopy, DGGE method with dinoflagellate specific primers demonstrated that Prorocentrum micans, Gymnodinium galatheanum and Gyrodinium uncatenum were also present during the bloom. Population shifts among the minor dinoflagellate groups were observed. DGGE of PCR-amplified 16S rRNA gene fragments indicated that cyanobacteria, α, β, γ-proteobacteria, FlavobacteriumBacteroides-Cytophaga (FBC), and Planctomcetes were the major components of bacterial assemblages during the bloom. DGGE analysis showed that Cytophagales and α-proteobacteria played important roles at different stages of dinoflagellates bloom. DGGE can be used as a rapid tool to simultaneously monitor population dynamics of both bacterial and dinoflagellates communities in aquatic environments, which is demonstrated here.  相似文献   

中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究   总被引：3，自引：1，他引：3  

孔晓瑜  喻子牛  刘亚军  高天翔  武云飞 《中国海洋大学学报(自然科学版)》2001,31(6):861-866

以相应引物 PCR扩增了黄河口中华绒螯蟹线粒体细胞色素氧化酶 I亚基基因 (COI)片段 ,PCR产物经 T载体连接之后进行克隆、测序 ,得到 70 9bp的碱基序列 ,其 A,T,G,C含量分别为 34.4 1% ,2 7.93% ,2 0 .0 3%和 17.6 3%。并比较它与珠江流域中华绒螯蟹 COI序列和日本绒螯蟹 COI序列的差异 ,发现黄河口中华绒螯蟹与珠江流域中华绒螯蟹 COI序列完全相同 ,而与日本绒螯蟹差异非常明显 ,70 9或 6 5 8(不计引物 )位点中核苷酸差异数为 32 ,核苷酸差异率为 4 .5 1%或 4 .86 % (不计引物 ) ,其中 2 5个位点为转换 ,7个位点为颠换。作者倾向于支持存在中华绒螯蟹和日本绒螯蟹 ,或它们为同一种的两个地理亚种的观点  相似文献   

Heuristic approaches for mine planning and production scheduling   总被引：4，自引：0，他引：4  

Mark Gershon 《Geotechnical and Geological Engineering》1987,5(1):1-13

Summary Traditional methods and opimization methods for mine planning and production scheduling have failed to provide easy to use tools that yield improved results. Two new approaches, each combining the best of the traditional and the optimization, are described.  相似文献   

高等学校毕业生分配的模糊数学方法     

胡继才 《武汉大学学报(信息科学版)》1989,(2)

本文用模糊数学原理建立了高等学校毕业生分配工作的数学模型,其中各因素的权重排序由层次分析法确立。通过梯形分布建立模糊分布,排除了各方面人为因素的干扰,并用实例说明了这种方法的可行性和可靠性。  相似文献